3,5-Dihydroxybenzoic acid is a potential biomarker of food intake like beer, nuts, peanuts and legumes.

Physicochemical Properties

Molecular Formula	C7H6O4
Molecular Weight	154.1201
Exact Mass	154.026
CAS #	99-10-5
Related CAS #	145639-71-0
PubChem CID	7424
Appearance	White to off-white solid powder
Density	1.6±0.1 g/cm3
Boiling Point	411.5±15.0 °C at 760 mmHg
Melting Point	236-238 °C (dec.)(lit.)
Flash Point	216.8±16.9 °C
Vapour Pressure	0.0±1.0 mmHg at 25°C
Index of Refraction	1.671
LogP	1.12
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Heavy Atom Count	11
Complexity	147
Defined Atom Stereocenter Count	0
InChi Key	UYEMGAFJOZZIFP-UHFFFAOYSA-N
InChi Code	InChI=1S/C7H6O4/c8-5-1-4(7(10)11)2-6(9)3-5/h1-3,8-9H,(H,10,11)
Chemical Name	3,5-dihydroxybenzoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Additional Infomation

3,5-dihydroxybenzoic acid is a dihydroxybenzoic acid in which the hydroxy groups are located at positions 3 and 5. It has a role as a metabolite. It is a dihydroxybenzoic acid and a member of resorcinols. It is functionally related to a benzoic acid. 3,5-Dihydroxybenzoic acid has been reported in Rubus niveus, Viburnum cylindricum, and other organisms with data available.

Solubility Data

Solubility (In Vitro)

H2O : ~60 mg/mL (~389.31 mM) DMSO : ~50 mg/mL (~324.42 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (16.22 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (16.22 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: 50 mg/mL (324.42 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.4885 mL	32.4423 mL	64.8845 mL
	5 mM	1.2977 mL	6.4885 mL	12.9769 mL
	10 mM	0.6488 mL	3.2442 mL	6.4885 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.