3,4-Methylenedioxycinnamic acid is an inhibitor (blocker/antagonist) of the phenylpropanoid enzyme 4-hydroxycinnamoyl-CoA ligase. 3,4-Methylenedioxycinnamic acid promotes the formation of soluble phenols, especially vanillic acid.

Physicochemical Properties

Molecular Formula	C10H8O4
Molecular Weight	192.1681
Exact Mass	192.042
CAS #	2373-80-0
PubChem CID	643181
Appearance	Off-white to light yellow solid powder
Density	1.4±0.1 g/cm3
Boiling Point	361.5±11.0 °C at 760 mmHg
Melting Point	242-244 °C (dec.)(lit.)
Flash Point	148.9±12.8 °C
Vapour Pressure	0.0±0.9 mmHg at 25°C
Index of Refraction	1.653
LogP	2.53
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	2
Heavy Atom Count	14
Complexity	249
Defined Atom Stereocenter Count	0
SMILES	C1OC2=C(O1)C=C(C=C2)/C=C/C(=O)O
InChi Key	QFQYZMGOKIROEC-DUXPYHPUSA-N
InChi Code	InChI=1S/C10H8O4/c11-10(12)4-2-7-1-3-8-9(5-7)14-6-13-8/h1-5H,6H2,(H,11,12)/b4-2+
Chemical Name	(E)-3-(1,3-benzodioxol-5-yl)prop-2-enoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Funk C, Brodelius PE. Phenylpropanoid Metabolism in Suspension Cultures of Vanilla planifolia Andr. : II. Effects of Precursor Feeding and Metabolic Inhibitors. Plant Physiol. 1990 Sep;94(1):95-101.
Additional Infomation	Methylenedioxycinnamic acid is a hydroxycinnamic acid. 3,4-Methylenedioxycinnamic acid has been reported in Brombya platynema and Helichrysum arenarium with data available.

Solubility Data

Solubility (In Vitro)

DMSO : ≥ 100 mg/mL (~520.37 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1 mg/mL (5.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1 mg/mL (5.20 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.2037 mL	26.0186 mL	52.0373 mL
	5 mM	1.0407 mL	5.2037 mL	10.4075 mL
	10 mM	0.5204 mL	2.6019 mL	5.2037 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.