2'-O-Methylcytidine is a 2'-generation nucleoside that can inhibit HCV (hepatitis C virus) replication. 2'-O-Methylcytidine inhibits NS5B-catalyzed RNA synthesis in vitro by competing with the substrate nucleoside triphosphate.

Physicochemical Properties

Molecular Formula	C10H15N3O5
Molecular Weight	257.24
Exact Mass	257.101
CAS #	2140-72-9
PubChem CID	150971
Appearance	White to off-white solid powder
Density	1.7±0.1 g/cm3
Boiling Point	506.0±60.0 °C at 760 mmHg
Melting Point	252-253ºC
Flash Point	259.8±32.9 °C
Vapour Pressure	0.0±3.0 mmHg at 25°C
Index of Refraction	1.677
LogP	-1.09
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	18
Complexity	397
Defined Atom Stereocenter Count	4
SMILES	CO[C@@H]1[C@@H]([C@H](O[C@H]1N2C=CC(=NC2=O)N)CO)O
InChi Key	RFCQJGFZUQFYRF-ZOQUXTDFSA-N
InChi Code	InChI=1S/C10H15N3O5/c1-17-8-7(15)5(4-14)18-9(8)13-3-2-6(11)12-10(13)16/h2-3,5,7-9,14-15H,4H2,1H3,(H2,11,12,16)/t5-,7-,8-,9-/m1/s1
Chemical Name	4-amino-1-[(2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxyoxolan-2-yl]pyrimidin-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Inhibition of hepatitis C virus RNA replication by 2'-modified nucleoside analogs. J Biol Chem. 2003 Apr 4;278(14):11979-84.
Additional Infomation	2'-O-methylcytidine is a methylcytidine.

Solubility Data

Solubility (In Vitro)	H2O : 62.5 mg/mL (242.96 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (388.74 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.8874 mL	19.4371 mL	38.8742 mL
	5 mM	0.7775 mL	3.8874 mL	7.7748 mL
	10 mM	0.3887 mL	1.9437 mL	3.8874 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.