2-Methoxybenzaldehyde (o-Anisaldehyde) is extracted from cinnamon essential oil (CEO) and has antibacterial and antifungal activity.

Physicochemical Properties

Molecular Formula	C8H8O2
Molecular Weight	136.15
Exact Mass	136.052
CAS #	135-02-4
Related CAS #	2-Methoxybenzaldehyde-d3;56248-49-8
PubChem CID	8658
Appearance	Colorless to light yellow <34°C powder,>40°C liquid
Density	1.1±0.1 g/cm3
Boiling Point	243.5±0.0 °C at 760 mmHg
Melting Point	34-40 °C(lit.)
Flash Point	117.8±0.0 °C
Vapour Pressure	0.0±0.4 mmHg at 25°C
Index of Refraction	1.547
LogP	1.72
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	2
Heavy Atom Count	10
Complexity	112
Defined Atom Stereocenter Count	0
SMILES	O(C([H])([H])[H])C1=C([H])C([H])=C([H])C([H])=C1C([H])=O
InChi Key	PKZJLOCLABXVMC-UHFFFAOYSA-N
InChi Code	InChI=1S/C8H8O2/c1-10-8-5-3-2-4-7(8)6-9/h2-6H,1H3
Chemical Name	2-methoxybenzaldehyde
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Synergistic effects of cinnamaldehyde and cinnamic acid in cinnamon essential oil against S. pullorum. Industrial Crops and Products, 2021-02-03. [2]. Interesting anticandidal effects of anisic aldehydes on growth and proton-pumping-ATPase-targeted activity. Microb Pathog. 2011 Oct;51(4):277-84.
Additional Infomation	2-Methoxybenzaldehyde is a carbonyl compound.

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (734.48 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (18.36 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (18.36 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (18.36 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	7.3448 mL	36.7242 mL	73.4484 mL
	5 mM	1.4690 mL	7.3448 mL	14.6897 mL
	10 mM	0.7345 mL	3.6724 mL	7.3448 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.