2-Bromoacetamide inactivates alcohol dehydrogenase.

Physicochemical Properties

Molecular Formula	C2H4BRNO
Molecular Weight	137.96
Exact Mass	136.947
CAS #	683-57-8
PubChem CID	69632
Appearance	White to off-white solid powder
Density	1.8±0.1 g/cm3
Boiling Point	271.6±23.0 °C at 760 mmHg
Melting Point	87-91 °C(lit.)
Flash Point	118.1±22.6 °C
Vapour Pressure	0.0±0.6 mmHg at 25°C
Index of Refraction	1.511
LogP	-0.52
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	1
Heavy Atom Count	5
Complexity	44.9
Defined Atom Stereocenter Count	0
SMILES	BrC([H])([H])C(N([H])[H])=O
InChi Key	JUIKUQOUMZUFQT-UHFFFAOYSA-N
InChi Code	InChI=1S/C2H4BrNO/c3-1-2(4)5/h1H2,(H2,4,5)
Chemical Name	2-bromoacetamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Fries RW, et al. Activation of liver alcohol dehydrogenases by imidoesters generated in solution. Biochemistry. 1975;14(23):5233‐5238.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (724.85 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (18.12 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (18.12 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (18.12 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	7.2485 mL	36.2424 mL	72.4848 mL
	5 mM	1.4497 mL	7.2485 mL	14.4970 mL
	10 mM	0.7248 mL	3.6242 mL	7.2485 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.