2,3-Bis(3-indolylmethyl)indole significantly inhibited RANKL-induced osteoclast formation, actin ring formation, and bone resorption in a concentration-dependent fashion.

Physicochemical Properties

Molecular Formula	C26H21N3
Molecular Weight	375.47
Exact Mass	375.174
CAS #	138250-72-3
PubChem CID	132142
Appearance	Light yellow to light brown solid powder
Density	1.314g/cm3
Boiling Point	682.1ºC at 760mmHg
Flash Point	306.6ºC
Vapour Pressure	1.09E-17mmHg at 25°C
Index of Refraction	1.796
LogP	6.312
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	0
Rotatable Bond Count	4
Heavy Atom Count	29
Complexity	564
Defined Atom Stereocenter Count	0
InChi Key	MJHVZTDHBWQIMN-UHFFFAOYSA-N
InChi Code	InChI=1S/C26H21N3/c1-4-10-23-19(7-1)17(15-27-23)13-22-21-9-3-6-12-25(21)29-26(22)14-18-16-28-24-11-5-2-8-20(18)24/h1-12,15-16,27-29H,13-14H2
Chemical Name	2,3-bis(1H-indol-3-ylmethyl)-1H-indole
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Anti-osteoclastogenic Effects of Indole Alkaloids Isolated from Barley (Hordeum vulgare Var. Hexastichon) Grass. J Agric Food Chem. 2021 Nov 10;69(44):12994-13005.

Solubility Data

Solubility (In Vitro)

DMSO :~100 mg/mL (~266.33 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.66 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6633 mL	13.3166 mL	26.6333 mL
	5 mM	0.5327 mL	2.6633 mL	5.3267 mL
	10 mM	0.2663 mL	1.3317 mL	2.6633 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.