2,3,5-Trimethylpyrazine is an endogenously produced metabolite.

Physicochemical Properties

Molecular Formula	C₇H₁₀N₂
Molecular Weight	122.17
Exact Mass	122.084
CAS #	14667-55-1
PubChem CID	26808
Appearance	Colorless to light yellow liquid
Density	1.0±0.1 g/cm3
Boiling Point	173.1±35.0 °C at 760 mmHg
Flash Point	54.4±0.0 °C
Vapour Pressure	1.7±0.3 mmHg at 25°C
Index of Refraction	1.504
LogP	1.1
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	0
Heavy Atom Count	9
Complexity	92.9
Defined Atom Stereocenter Count	0
SMILES	N1C(C([H])([H])[H])=C([H])N=C(C([H])([H])[H])C=1C([H])([H])[H]
InChi Key	IAEGWXHKWJGQAZ-UHFFFAOYSA-N
InChi Code	InChI=1S/C7H10N2/c1-5-4-8-6(2)7(3)9-5/h4H,1-3H3
Chemical Name	2,3,5-trimethylpyrazine
Synonyms	2,3,5Trimethylpyrazine; 2,3,5 Trimethylpyrazine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Additional Infomation

Trimethylpyrazine is a member of the class of pyrazines that is pyrazine in which three hydrogens at positions 2, 3 and 5 have been replaced by methyl groups. It has a role as a plant metabolite, a flavouring agent, a bacterial metabolite, an animal metabolite and a pheromone. 2,3,5-Trimethylpyrazine has been reported in Camellia sinensis, Streptomyces antioxidans, and other organisms with data available. Trimethylpyrazine is a metabolite found in or produced by Saccharomyces cerevisiae.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~818.53 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (20.46 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (20.46 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	8.1853 mL	40.9266 mL	81.8532 mL
	5 mM	1.6371 mL	8.1853 mL	16.3706 mL
	10 mM	0.8185 mL	4.0927 mL	8.1853 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.