PW0464 is a full G protein ligand with nanomolar activity and an agonist of D1R with EC50 of 5.8 nM (Gs-cAMP).

Physicochemical Properties

Molecular Formula	C19H17F2N3O4
Molecular Weight	389.35
Exact Mass	389.118
CAS #	1643462-93-4
Related CAS #	Razpipadon;1643489-35-3
PubChem CID	86764103
Appearance	White to off-white solid powder
LogP	3.3
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	5
Heavy Atom Count	28
Complexity	643
Defined Atom Stereocenter Count	0
InChi Key	ZXIPVZWZRQCIRW-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H17F2N3O4/c1-10-9-12(27-17-14(28-18(20)21)5-4-8-22-17)6-7-13(10)15-11(2)16(25)23-19(26)24(15)3/h4-9,18H,1-3H3,(H,23,25,26)
Chemical Name	6-[4-[3-(difluoromethoxy)pyridin-2-yl]oxy-2-methylphenyl]-1,5-dimethylpyrimidine-2,4-dione
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	PW0464 (compound 24) exhibits no action for D1R-mediated β-arrestin recruitment and complete G protein bias[1]. Compound 24, or PW0464, is a non-catechol agonist that forms connections with S2025 and S1985.42. 46 via its atom of fluorine[2].
References	[1]. Pingyuan Wang, et al. Synthesis and Pharmacological Evaluation of Noncatechol G Protein Biased and Unbiased Dopamine D1 Receptor Agonists. ACS Med Chem Lett [2]. David R Sibley, et al. Novel Cryo-EM structures of the D1 dopamine receptor unlock its therapeutic potential. Signal Transduct Target Ther. 2021 May 22;6(1):205.

Solubility Data

Solubility (In Vitro)

DMSO: 25 mg/mL (64.21 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.42 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.5684 mL	12.8419 mL	25.6838 mL
	5 mM	0.5137 mL	2.5684 mL	5.1368 mL
	10 mM	0.2568 mL	1.2842 mL	2.5684 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.