| Description | TMRE (Tetramethylrhodamine ethyl ester perchlorate) is a mitochondria specific dye (λex: 550 nm, λem: 575 nm). |
| In vitro | 在上皮细胞中观察到TMRE的多向动态移动，而在活体牛透镜的表层皮质纤维细胞中观察到双向动态移动。在上皮层中，TMRE荧光的移动速度可达5μm/min；在表层皮质中，观察到的移动速度高达18.5μm/min。使用解偶联剂碳酰氰化m-氯苯基肼（carbonyl cyanide m-chlorophenylhydrazone）[2]处理后，TMRE荧光的移动被抑制。 |
| Synonyms | 四甲基若丹明乙酯酸铵, Tetramethylrhodamine ethyl ester perchlorate |
| molecular weight | 514.95 |
| Molecular formula | C26H27ClN2O7 |
| CAS | 115532-52-0 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 27.78 mg/mL (53.95 mM), Sonication is recommended. |
| References | 1. Crowley LC, et al. Measuring Mitochondrial Transmembrane Potential by TMRE Staining. Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.prot087361. 2. Bantseev V, et al. Confocal laser scanning microscopy imaging of dynamic TMRE movement in the mitochondria of epithelial and superficial cortical fiber cells of bovine lenses. Mol Vis. 2005 Jul 14;11:518-23. |