| Description | Tenofovir Disoproxil (GS 4331) is a nucleotide reverse transcriptase inhibitor to treat HIV and chronic Hepatitis B. |
| Cell experiments | Human immortalized epithelial HK-2 cells were grown in a keratinocyte-free media with 50 μg/mL bovine pituitary extract and 5 ng/mL recombinant epithelial growth factor from Invitrogen. Cells were grown in a warm humidified incubator with constant settings of 37 °C and 5% CO2. HK-2 cells were plated into six-well tissue culture plates (750,000 cells/mL) and allowed to grow for 48 h. Media was replaced and cells were treated with a final concentration of 0, 1.5, 3.0, 4.75, 14.5, or 28.8 μM TFV for 24, 48, or 72 h. The vehicle was an equal volume of phosphate buffered saline (PBS). Abacavir was prepared in sterile water and cells were treated for 24 h with 0, 1.5, 3 or 6 μM of abacavir to evaluate renal sensitivity to an agent recognized to be less nephrotoxic. Following the treatment period, cells were collected with Trypsin-EDTA (0.25%) for sample analysis[1]. |
| Synonyms | 替诺福韦酯, GS 4331, Bis(POC)-PMPA |
| molecular weight | 519.44 |
| Molecular formula | C19H30N5O10P |
| CAS | 201341-05-1 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 45 mg/mL (86.63 mM), Sonication is recommended. |
| References | 1. Rachel M , Reagan S , Brooke P , et al. Establishment of HK-2 Cells as a Relevant Model to Study Tenofovir-Induced Cytotoxicity[J]. International Journal of Molecular Sciences, 2017, 18(3):531-. 2. Menne S , Cote P J , Korba B E , et al. Antiviral Effect of Oral Administration of Tenofovir Disoproxil Fumarate in Woodchucks with Chronic Woodchuck Hepatitis Virus Infection[J]. Antimicrobial Agents and Chemotherapy, 2005, 49(7):2720-2728. 3. Fical L. Vývoj UHPLC-MS/MS metody pro analýzu vybraných antivirotik v HILIC a RP módu[J]. 2020 4. Fical L, Khalikova M, Kočová Vlčková H, et al. Determination of Antiviral Drugs and Their Metabolites Using Micro-Solid Phase Extraction and UHPLC-MS/MS in Reversed-Phase and Hydrophilic Interaction Chromatography Modes[J]. Molecules. 2021, 26(8): 2123. 5. Thieulent C, Hue E, Sutton G, et al. Identification of antiviral compounds against equid herpesvirus-1 using real-time cell assay screening: efficacy of decitabine and valganciclovir alone or in combination[J]. Antiviral Research. 2020: 104931 |
| Citations | 1. Thieulent C, Hue E, Sutton G, et al. Identification of antiviral compounds against equid herpesvirus-1 using real-time cell assay screening: efficacy of decitabine and valganciclovir alone or in combination. Antiviral Research. 2020: 104931 2. Fical L, Khalikova M, Kočová Vlčková H, et al. Determination of Antiviral Drugs and Their Metabolites Using Micro-Solid Phase Extraction and UHPLC-MS/MS in Reversed-Phase and Hydrophilic Interaction Chromatography Modes. Molecules. 2021, 26(8): 2123. |