| Description | SB-3CT is a potent and specific gelatinase inhibitor for MMP-2(Ki=13.9 nM) and MMP-9(Ki=600 nM). |
| In vitro | 在栓子引起的永久性脑缺血小鼠模型中,SB-3CT通过阻碍神经元层粘连蛋白的退化,保护神经元免受缺血性细胞死亡的损害.在L-CI.5sT细胞淋巴小鼠模型中,每天腹腔注射 SB-3CT（5-50 mg/kg）,能够抑制肝癌细胞转移.在人前列腺癌细胞骨转移模型中,每天腹腔注射SB-3CT（50 mg/kg ）,抑制细胞的生长,骨质溶解以及血管生成. |
| In vivo | SB-3CT能够降低溶骨性反应,有助于骨完整性的保持。在PC3肿瘤细胞中,SB-3CT抑制细胞的生长。在基底膜基质中,SB-3CT直接抑制骨髓内皮细胞浸润和小管形成。 |
| Cell experiments | SB-3CT is dissolved in DMSO (10 mM) and stored (-22°C), and then diluted with appropriate media (DMSO 1%) before use[2]. PC3 cells are seeded in 35-mm dishes (5×104 cells/dish) in complete culture medium. The next day, the medium is replaced with complete medium supplemented with 1% DMSO alone (vehicle) or SB-3CT (final concentrations 0.1-50 μM) in 1% DMSO. At various times, the cells are harvested with trypsin and counted[2]. |
| Target activity | MMP2:13.9 nM(Ki), MMP9:600 nM(Ki) |
| molecular weight | 306.4 |
| Molecular formula | C15H14O3S2 |
| CAS | 292605-14-2 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | Ethanol: 6.1 mg/mL (20 mM) DMSO: 55 mg/mL (179.5 mM) |
| References | 1. Brown S, et al. J Am Chem Soc. 2000, 122 (28), 6799–6800. 2. Krüger A, et al. Cancer Res. 2005, 65(9), 3523-3526 3. Bonfil RD, et al. Int J Cancer. 2006, 118(11), 2721-2726. 4. Cui J, et al. Mol Neurodegener. 2012, 15, 7-21. 5. Lei Z, Jian M, Wei J, et al. Array-Based in situ Fluorescence Assay for Profiling Multiplex Matrix Metalloproteinases Activities in Tissue Section[J]. Analytica Chimica Acta. 2019. 6. Lei Z, Chen H, Zhang H, et al. Evaluation of Matrix Metalloproteinase Inhibition by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Substrate and in Vivo Assessment[J]. ACS applied materials & interfaces. 2017 Dec 20;9(50):44241-44250. |
| Citations | 1. Lei Z, Chen H, Zhang H, et al. Evaluation of Matrix Metalloproteinase Inhibition by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Substrate and in Vivo Assessment. ACS applied materials & interfaces. 2017 Dec 20;9(50):44241-44250. 2. Lei Z, Jian M, Wei J, et al. Array-Based in situ Fluorescence Assay for Profiling Multiplex Matrix Metalloproteinases Activities in Tissue Section. Analytica Chimica Acta. 2019 3. Liu H, Yao C, Zhang L, et al. Nanoliposomes co-encapsulating Ce6 and SB3CT against the proliferation and metastasis of melanoma with the integration of photodynamic therapy and NKG2D-related immunotherapy on A375 cells. Nanotechnology. 2021 |