| Description | RP-54745, a potential antirheumatic compound. Inhibitor of macrophage stimulation and interleukin-1 production. |
| In vivo | RP-54745可能会降低LPS诱导的小鼠腹膜巨噬细胞产生的白细胞介素-1（IL-1）[1]，同时，RP-54745可能对调节免疫系统的细胞和细胞因子产生影响，其功能失调可能导致炎症性疾病或自体免疫性病理变化[2]。 |
| Animal experiments | 1.5*10^6 peritoneal murine macrophages, after a 2-h period of adherence, were cultured in RPMI 1640 with 5% FCS for 20 h at 37 ℃ in 5% CO2.?In vitro, test compounds were added 15 min before the stimulant, 100ng/ml LPS, and the cells were incubated for 2-24 h. In vivo, thioglycollate-stimulated female OFt mice were orally treated with 5 or 25 mg/kg/day RP 54745 for 4 days.?Harvested macrophages were then stimulated ex vivo for 2 h with LPS at concentrations ranging from 0.1-10 ng/ml.?In some assays, 4 h before macrophage harvesting, mice were treated intraperitoneally with LPS: 0.5 ug per mouse.?At the end of the in vitro incubation period, supernatants were removed from cultures, and cells were lysed by three freeze/thaw cycles.?Both extracellular and cell-associated IL-1 activities were assayed. |
| molecular weight | 297.82 |
| Molecular formula | C13H12ClNOS2 |
| CAS | 135330-08-4 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 62.5 mg/mL (209.86 mM) |
| References | 1. Folliard F , Bousseau A , Terlain B . RP 54745, a potential antirheumatic compound. I. Inhibitor of macrophage stimulation and interleukin-1 production[J]. Agents and Actions, 1992, 36(1-2):119-126. 2. Folliard F , Bousseau A , Terlain B . RP 54745, a potential antirheumatic compound. II. In vivo properties in different animal models[J]. Agents and Actions, 1992, 36(1-2):127-135. |