| Description | RJW100 is a compound that acts as a potent agonist of liver receptor homolog 1 (LRH-1, NR5A2) and steroidogenic factor-1 (SF-1, NR5A1), exhibiting pEC50 values of 6.6 and 7.5, respectively. Additionally, RJW100 induces robust activation of the miR-200c (miRNA-200c, microRNA-200c) promoter. |
| In vitro | RJW100（compound 24-exo）处理能显著地、剂量依赖性地增加小/短异源二聚体伙伴（SHP）转录本，起始浓度为5 µM。在1 µM的剂量下，RJW100（compound 24-exo）几乎完全置换SF-1上已结合的PIP2磷脂。使用未与任何磷脂复合的hLRH-1 LBD单独测试时，结果显示RJW100（compound 24-exo; 0-100 µM）结合后，hLRH-1 LBD（配体结合域）在天然PAGE迁移上表现出明确的剂量依赖性转变[1]。RJW100强烈激活miR-200c启动子，并展现出强大的下调ZEB1和ZEB2蛋白的能力[2]。 |
| molecular weight | 386.57 |
| Molecular formula | C28H34O |
| CAS | 1276664-20-0 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 55 mg/mL (142.27 mM) |
| References | 1. Richard J Whitby, et al. Small molecule agonists of the orphan nuclear receptors steroidogenic factor-1 (SF-1, NR5A1) and liver receptor homologue-1 (LRH-1, NR5A2). J Med Chem. 2011 Apr 14;54(7):2266-81. 2. Yuxia Zhang, et al. Regulation of miR-200c by nuclear receptors PPARα, LRH-1 and SHP. Biochem Biophys Res Commun. 2011 Dec 9;416(1-2):135-9. |