| Description | Pyranine (Solvent Green 7) is reported as a new class of fluorescent chemosensor for the Cu+ ion. |
| In vitro | Pyranine (Py) 是一种荧光探针[3]，能够在竞争环境中区分Cu+离子和其他范围的阳离子。Pyranine 对Cu+离子显示出快速的荧光响应（t1/2=1.66分钟），其μM级的检测限使得在环境样品中检测此离子成为可能。Pyranine与Cu+之间的络合物配比为2:1[1]。通过测量两个激发波长下的荧光强度比I463/I404来计算pH值，该比率依赖于pH值，但不受荧光团浓度和其他可能改变强度的变量的影响。在兔子眼中，观察到角膜和前房内的Pyranine在眼睑闭合和佩戴隐形眼镜时容易测量到与pH轻微变化相关的荧光比率变化，表明其对pH的轻微变化非常敏感[2]。 |
| Synonyms | 溶剂绿7, HPTS, Solvent Green 7 |
| molecular weight | 524.39 |
| Molecular formula | C16H7Na3O10S3 |
| CAS | 6358-69-6 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: 100 mg/mL (190.70 mM), Sonication is recommended. DMSO: 6.88 mg/mL (13.11 mM), Sonication is recommended. |
| References | 1. Saha T, et al. In vitro sensing of Cu(+) through a green fluorescence rise of pyranine. Photochem Photobiol Sci. 2014 Oct;13(10):1427-33. 2. Thomas JV, et al. The fluorescent indicator pyranine is suitable for measuring stromal and cameral pH in vivo. Exp Eye Res. 1990 Mar;50(3):241-9. 3. Evingür GA, et al. Kinetic models for the dynamical behavior of polyacrylamide (PAAm)-κ-carrageenan (κC) composite gels. J Biol Phys. 2015 Jan;41(1):37-47. 4. Ge S, Wang X, Hou Y, et al. Repositioning of histamine H1 receptor antagonist: Doxepin inhibits viropexis of SARS-CoV-2 Spike pseudovirus by blocking ACE2[J]. European Journal of Pharmacology. 2021: 173897. |
| Citations | 1. Yu, W., et al. AMPK-SIRT1 Pathway Modulates The Apoptosis, Proliferation and Migration of AR42J Cells By Regulating p53 and NF-κB. European Journal of Pharmacology. 2021 2. Ge S, Wang X, Hou Y, et al. Repositioning of histamine H1 receptor antagonist: Doxepin inhibits viropexis of SARS-CoV-2 Spike pseudovirus by blocking ACE2. European Journal of Pharmacology. 2021 Apr 5;896:173897. doi: 10.1016/j.ejphar.2021.173897. Epub 2021 Jan 23. |