| Description | PKM2-IN-1 (compound 3k) displays PKM2 inhibitory activity with the IC50 value of 2.95 μM. The IC50 value for PKM1 is 4-5-fold higher than that for PKM2. |
| In vitro | Results show that most of the tested compounds exhibit some degree of PKM2 inhibition and some compounds, such as PKM2-IN-1 (compound 3k) and 6d, display more potent activity than the positive control shikonin. The representative compounds PKM2-IN-1, 6d display dose-dependent inhibition of PKM2 with less inhibition of PKM1 and PKL like shikonin. Among all tested compounds, the most potent compounds are 3a, PKM2-IN-1 and 3r, which exhibit IC50 values against HCT116 and Hela cells ranging from 0.39 to 0.41 μM, 0.18 to 0.29 μM and 0.18 to 0.38 μM, respectively. |
| Cell experiments | 在 THP-1 细胞中，使用WB检测抑制或激活 PKM2 后 p-STAT3 的表达情况，用 PKM2-IN-1 (T4170, 10 μM) 和 PKM2 activator 2 (T62758, 100 nM) 处理 48 h。 |
| Target activity | PKM2:2.95 μM |
| Synonyms | PKM2 inhibitor, compound 3k |
| molecular weight | 345.48 |
| Molecular formula | C18H19NO2S2 |
| CAS | 94164-88-2 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 1 mg/ml, Sonication is recommended. Ethanol: < 1 mg/mL (insoluble or slightly soluble) H2O: < 1 mg/mL (insoluble or slightly soluble) |
| References | 1. Ning X, et al. Discovery of novel naphthoquinone derivatives as inhibitors of the tumor cell specific M2 isoform of pyruvate kinase. Eur J Med Chem. 2017 Sep 29;138:343-352. |
| Citations | 1. Deng H, Qian X, Zhang Y, et al.Metformin Increases the Response of Cholangiocarcinoma Cells to Gemcitabine by Suppressing Pyruvate Kinase M2 to Activate Mitochondrial Apoptosis.Digestive Diseases and Sciences.2024: 1-15. |