| Description | Neocuproine is a copper(I) chelator that enhances the purinergic component of vasoconstriction induced by electric field stimulation, and is often used as a ligand reagent and copper ion detector.Neocuproine forms stable complexes with copper ions and can play a catalytic role in certain chemical reactions and analytical methods.Neocuproine acts as a redox-active on the iron and cobalt ligand platform for protection against oxidative damage in NSC34 cells. |
| In vitro | Neocuproine (100 lM) 通常抑制炎症介质的生成。[2] Neocuproine 处理降低了 IFN-γ、MCP-1、MCP-3 和 VEGF-A 的水平。KC/GRO 的产生由于 Neocuproine 缺乏而下调。[2] 与 ATP7A 缺乏不同，Neocuproine 减少了 FGF-9、IL-1α、IL-12p70、IL-2、IL-3、IL-4、IL-6、MIP-1β、MIP-2、RANTES 和 TNFα 的产生。[2] |
| In vivo | Neocuproine (100微摩) 显著抑制了去卵巢非妊娠大鼠子宫自发收缩的幅度和频率，而该化合物却能在妊娠大鼠和人类子宫中促进自发或催产素诱导的收缩频率，但不改变这些收缩的幅度。Neocuproine (200微摩) 能增强妊娠子宫收缩的幅度。这些效应被嘌呤类受体拮抗剂舒拉明 (100微摩) 阻断，并且在给予neocuproine-铜(I) 复合物或铜(II) 螯合剂cuprizone后不发生。alpha, beta-甲烷基ATP增加了妊娠子宫收缩的幅度和频率，但对去卵巢非妊娠大鼠子宫的收缩无影响，而neocuproine增强了这种促进效应。 |
| Synonyms | Neocuproin |
| molecular weight | 208.26 |
| Molecular formula | C14H12N2 |
| CAS | 484-11-7 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 70 mg/mL (336.12 mM), Sonication is recommended. |
| References | 1. Castro PA, et al. Copper-uptake is critical for the down regulation of synapsin and dynamin induced by neocuproine: modulation of synaptic activity in hippocampal neurons. Front Aging Neurosci. 2014 Dec 3;6:319. 2. Patel OV, et al. Production of LPS-induced inflammatory mediators in murine peritoneal macrophages: neocuproine as a broad inhibitor and ATP7A as a selective regulator. Biometals. 2013 Jun;26(3):415-25. 3. Kumcu EK, et al. Differential effect of neocuproine, a copper(I) chelator, on contractile activity in isolated ovariectomized non-pregnant rat, pregnant rat and pregnant human uterus. Eur J Pharmacol. 2009 Mar 1;605(1-3):158-63. 4. Soares SAR, et al. Comparison of Spectrophotometric Methods for the Determination of Copper in Sugar Cane Spirit. J AOAC Int. 2018 May 1;101(3):876-882. |