| Description | N1-Methylpseudouridine (1-Methylpseudouridine) is a methylpseudouridine and enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density in mRNA. |
| In vitro | 在HEK293T细胞中，将N1-Methylpseudouridine纳入mRNA可以改变所产生的mRNA，使之产生的luc量高于标准Luc mRNA。N1-Methylpseudouridine核苷改造在Luc和GFP mRNA中的引入，部分通过抑制eIF2α磷酸化，增强了翻译启动步骤。此外，由于延长速率的减少，含N1-Methylpseudouridine的Luc mRNA的多聚体形成和生长得到了增强[1]。 |
| In vivo | 含有N1-Methylpseudouridine改性的mRNA单独或与5-甲基胞嘧啶(m5C)结合使用，在转染至细胞系或小鼠后，相较于当前最先进的伪尿苷(Ψ)和/或m5C/Ψ改性mRNA平台，表现出显著更高的报告基因表达能力，双重改性mRNA的表达提升达到约44倍，单一改性mRNA的提升约为13倍。研究表明，(m5C/)N1-Methylpseudouridine改性mRNA在体外转染时，相比于(m5C/)Ψ改性mRNA，能够减少细胞内固有免疫原性，并提高细胞活力。 |
| Synonyms | N1-甲基-假尿苷, 1-Methylpseudouridine |
| molecular weight | 258.23 |
| Molecular formula | C10H14N2O6 |
| CAS | 13860-38-3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: 40 mg/mL (154.90 mM) DMSO: 100 mg/mL (387.25 mM) |
| References | 1. Svitkin YV, et al. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density. Nucleic Acids Res. 2017 Jun 2;45(10):6023-6036. 2. Andries O, et al. N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. J Control Release. 2015 Nov 10;217:337-44. |