| Description | L 012 sodium salt, a luminol analogue, is a widely used chemiluminescent (CL) probe for active oxygen and active nitrogen. L 012 sodium salt is widely used to detect the superoxides (O2•−) derived from NADPH oxidase (Nox) to find Nox inhibitors. |
| In vitro | L 012 sodium salt是一种新型化学发光探针。在产生活性氧物种(ROS)的激活EoL-1细胞中，L-012的处理产生了明显的化学发光。而超氧化物歧化酶（100-300 U/ml）消除了化学发光，这表明主要检测到的ROS是O2- [1]。在培养的内皮细胞中，佛波醇12-肉豆蔻酸13-酯（PMA，1 μM）使L-012的化学发光增加了2.8倍。钒酸盐（100 μM）将化学发光增加至17倍。这些结果表明，由NAD(P)H氧化酶产生的超氧增加。 |
| In vivo | 在人体口腔、血液以及大鼠腹膜腔中，L-012与活化中性粒细胞产生的ROS发生反应，产生了强烈的化学发光，其亮度高于MCLA [2]。在非肥胖糖尿病小鼠和K8敲除（K8(-/-)）小鼠中，通过给予硫酸右旋糖醇的处理，增加了L-012的化学发光，用于检测反应性氧和氮种类（ROS和RNS）[3]。 |
| molecular weight | 310.67 |
| Molecular formula | C13H8ClN4NaO2 |
| CAS | 143556-24-5 |
| Storage | |Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 3.0 mg/mL (9.6 mM), Sonication and heating to 60℃ are recommended. DMSO: 45.0 mg/mL (144.8 mM), Sonication is recommended. |
| References | 1. Sohn HY, et al. Sensitive superoxide detection in vascular cells by the new chemiluminescence dye L-012. J Vasc Res. 1999;36(6):456-464. 2. Imada I, et al. Analysis of reactive oxygen species generated by neutrophils using a chemiluminescence probe L-01Anal Biochem. 1999;271(1):53-58. 3. Asghar MN, et al. In vivo imaging of reactive oxygen and nitrogen species in murine colitis. Inflamm Bowel Dis. 2014;20(8):1435-1447. |