| Description | Ionomycin is an calcium ionophore and an antibiotic that binds calcium ions (Ca2+). It is produced by the bacterium Streptomyces conglobatus.It is used in research to raise the intracellular calcium level (Ca2+) and as a research tool to understand Ca2+ transport across biological membranes. Ionomycin promotes apoptosis and induces the activation of protein kinase C (PKC). |
| In vitro | Ionomycin (2 μM) induced cell death in LCLC 103H cells, derived from a human large cell lung carcinoma, by inducing calpain activation promotes decrease of Bcl-2 proteins thereby triggering the intrinsic apoptotic pathway [2].Ionomycin also phosphorylate p38 MAPK by Ca2+ influx through SOCE, leading to suppression of TNF-α-induced NF-κB phosphorylation[3]. |
| molecular weight | 709.01 |
| Molecular formula | C41H72O9 |
| CAS | 56092-81-0 |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | Ethanol: 90.0 mg/mL (126.9 mM), Sonication is recommended. DMSO: 90.0 mg/mL (126.9 mM), Sonication is recommended. |
| References | 1. Lautens M, et al. Total synthesis of ionomycin using ring-opening strategies. Org Lett. 2002 May 30;4(11):1879-82. 2. Assfalg-Machleidt I,et al. Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members. J Biol Chem. 2002 Jul 26;277(30):27217-26. 3. Uwada J, et al. Store-operated calcium entry (SOCE) contributes to phosphorylation of p38 MAPK and suppression of TNF-α signalling in the intestinal epithelial cells. Cell Signal. 2019 Nov;63:109358. |
| Citations | 1. Zhang Y, Shi Q, Wang P, et al.iPSC‐derived NK cells with site‐specific integration of CAR19 and IL24 at the multi‐copy rDNA locus enhanced antitumor activity and proliferation.MedComm.2024, 5(5): e553. |