| Description | HyT36 is a small molecule hydrophobic tag that selectively destabilizes and promotes the degradation of fusion proteins and the pseudokinase Her3. HyT36 can treat cells expressing erht induced acutely. |
| In vitro | HyT36 (10 µ M ; 24 h) reduced the expression of GFP-HaloTag2/Fz4-HaloTag2/GFP-HaloTag7 fusion protein in HEK293 cells.[1] HyT36 (10 µ M ; 2 or 48 h) reduced the expression of EGFP in HEK293 Flp-In cells expressing HA-EGFP-HaloTag2 and enhanced the binding of Hsp70 to the HA-EGFP-HaloTag2 fusion protein.[3] HyT36 (50 nM ; 24 h) reduced the content of EGFP HaloTag2 in HEK293 Flp In cells expressing HA-EGFP HaloTag2.[3] HyT36 (1 µ M ; 4 d) reduced the expression of HA HaloTag2 HRasG12V in NIH-3T3 cells expressing HA HaloTag2 HRasG12V.[3] |
| molecular weight | 442.07 |
| Molecular formula | C25H44ClNO3 |
| CAS | 1323151-45-6 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 90.0 mg/mL (203.6 mM ), Sonication is recommended. |
| References | 1. Tae HS, et al. Identification of hydrophobic tags for the degradation of stabilized proteins. Chembiochem. 2012 ; 13(4):538-541. 2. Rubner S, et al. Poloxin-2HT+: changing the hydrophobic tag of Poloxin-2HT increases Plk1 degradation and apoptosis induction in tumor cells. Org Biomol Chem. 2019 ; 17(12):3113-3117. 3. Neklesa TK, et al. A bidirectional system for the dynamic small molecule control of intracellular fusion proteins. ACS Chem Biol. 2013 ; 8(10):2293-2300. |