| Description | Hoechst 34580 tetrahydrochloride, a cell-permeable fluorescent dye, is used for staining DNA and nuclei. |
| In vitro | Hoechst 34580 is a good candidate for treating Alzheimer's disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37 °C for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose-dependent manner. The IC50 is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which gave 0.86±0.05 μM for Hoechst 34580. |
| Synonyms | Hoechst 34580 tetrahydrochloride (23555-00-2 free base), HOE 34580 tetrahydrochloride |
| molecular weight | 597.41 |
| Molecular formula | C27H33Cl4N7 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 41.2 mg/mL (68.96 mM), Sonication and heating are recommended. |
| References | 1. Thai NQ, et al. Discovery of DNA dyes Hoechst 34580 and 33342 as good candidates for inhibiting amyloid beta formation: in silico and in vitro study. J Comput Aided Mol Des. 2016 Aug;30(8):639-50. |