| Description | GRGDSPK acetate shows inhibitory activity against integrin-fibronectin binding and can be used in research on integrins in bone formation and resorption. |
| In vitro | 通过测量孕龄Sprague-Dawley大鼠的顶骨组织中的钙含量和70 bonehnit面积，我们发现GRGDSPK（EMD 56574; RGD; 0.1-50 μM; 持续4天）以剂量依赖的方式抑制矿化作用。[1] 经GRGDSPK处理（10和50 μM; 4天）后，骨骼形态出现显著改变，特征为成骨细胞和矿化基质的组织结构遭到破坏。[1] 在含GRGDSPK肽段（RGD; 250 μM）的培养基中，整合素与纤维连接蛋白之间的结合被有效阻断，导致野生型细胞的细胞聚集体平均大小显著增加。[2] 添加GRGDSPK（250 μM）显著减少了野生型中胚层前体细胞的黏附力和所施加的工作量，指出这些前体细胞表达的整合素的参与，以及记录的对纤维连接蛋白的脱附参数的特异性。[2] GRGDSPK（含RGD，1.5 mM、1.0 mM 和 0.5 mM）及RGD修饰的肽段以1.5 mM、1.0 mM 和 0.5 mM 的浓度，削弱了牛卵母细胞的受精能力，导致精子细胞的裂解率以剂量依赖的方式减少。[3] |
| Synonyms | GRGDSPK acetate(111119-28-9 Free base) |
| molecular weight | 775.81 |
| Molecular formula | C30H53N11O13 |
| CAS | 144027-77-0 |
| Storage | store at low temperature,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 7.76 mg/mL (10 mM) |
| References | 1. Gronowicz GA, et al. Synthetic peptide containing Arg-Gly-Asp inhibits bone formation and resorption in a mineralizing organ culture system of fetal rat parietal bones. J Bone Miner Res. 1994;9(2):193-201. 2. Puech PH, et al. Measuring cell adhesion forces of primary gastrulating cells from zebrafish using atomic force microscopy. J Cell Sci. 2005;118(Pt 18):4199-4206. 3. Sessions BR, et al. Effects of amino acid substitutions in and around the arginine-glycine-aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes. Mol Reprod Dev. 2006;73(5):651-657. |