| Description | β-Amyloid (25-35) (Aβ25-35) is the fragment Aβ(25-35) of the Alzheimer's amyloid β-peptide. Which has shown neurotoxic activities in cultured cells. |
| In vitro | β-Amyloid (25-35) 的氨基酸序列为NH2-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH，首个Gly代表第25个氨基酸，末尾的Met代表第35个氨基酸。本研究首次探讨了该多肽在凝胶状态下的特性。研究表明，Amyloid beta-peptide(25-35)能够对分离出的脑线粒体产生凋亡效应，其中methionine-35的氧化还原状态对于程序性细胞死亡途径及其毒性事件的诱导起着关键作用[2]。同时，通过振动吸收光谱和ECD进行了比较研究。此外，利用振动吸收光谱和VCD光谱技术也对Aβ(25-35)多肽膜的构象偏好进行了探究[1]。 |
| In vivo | Alzheimer氏病(AD)模型组显示出空间学习和记忆能力受损。CDS和多奈哌齐的给药显著改善了β-Amyloid (25-35)引起的记忆障碍，在Morris水迷宫(P<0.05)和被动避让任务(P<0.01)中与AD模型组相比有显著差异[3]。将β-Amyloid (25-35)通过脑室内注射给大鼠，随后每天给予丹参(CDS)一次，持续23天。采用Morris水迷宫和被动避让任务监测大鼠的行为。通过实时PCR和Western blotting技术检测海马中的淀粉前体蛋白(APP)、β-淀粉样蛋白前体裂解酶-1(BACE1)、前蛋白酶体-1(PS1)、胰岛素降解酶(IDE)和内肽酶(NEP)的表达。 |
| Synonyms | β-Amyloid peptide(25-35), Aβ25-35, BETA-淀粉样蛋白片断25-35, Amyloid beta-peptide(25-35) |
| molecular weight | 1060.27 |
| Molecular formula | C45H81N13O14S |
| CAS | 131602-53-4 |
| Storage | keep away from moisture|Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 3.33 mg/mL (3.14 mM), Sonication is recommended. DMSO: 97 mg/mL (91.49 mM), Sonication is recommended. |
| References | 1. D'Ursi AM, et al. Solution structure of amyloid beta-peptide (25-35) in different media. J Med Chem. 2004 Aug 12;47(17):4231-8. 2. Clementi ME, et al. Abeta(31-35) and Abeta(25-35) fragments of amyloid beta-protein induce cellular death throughapoptotic signals: Role of the redox state of methionine-35. FEBS Lett. 2005 May 23;579(13):2913-8. 3. Liu M, et al. Cognitive improvement of compound danshen in an Aβ25-35 peptide-induced rat model of Alzheimer's disease. BMC Complement Altern Med. 2015 Oct 23;15:382. |