| Description | FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone) is an oxidative phosphorylation (OXPHOS) inhibitor and mitochondrial proton carrier uncoupler. FCCP is often used as an apoptosis inducer. |
| In vitro | 方法：大鼠心室肌细胞用 FCCP (10-1000 nM) 处理 1400 sec，使用 Clark Oxygen Electrode 检测细胞耗氧量。结果：添加 FCCP 后，耗氧量明显、立即且呈剂量依赖性增加。[1]方法：大鼠肾上腺髓质嗜铬瘤细胞 PC12 用 FCCP (30 µM) 处理 0.5-2 h，使用 [3H]methionine 检测蛋白质合成速率。结果：FCCP 处理对蛋白质合成率产生了强烈的抑制作用 (68%)，持续处理至少 2 小时。[2] |
| In vivo | 方法：为检测对中风的影响，将 FCCP (1 mg/kg) 腹腔注射给中风的 C57BL/6J 小鼠模型，然后进行一小时的短暂性大脑中动脉闭塞 (tMCAO)。结果：用 FCCP 预处理的小鼠皮层、纹状体和全半球的梗死体积显著增加。与载体相比，接受 FCCP 的小鼠具有显著增加的神经功能缺损评分。[3]方法：为检测体内抗肿瘤活性，将 FCCP (1 mg/kg) 和 cisplatin (2 mg/kg) 腹腔注射给携带小鼠卵巢上皮癌肿瘤 ID8 的 C57BL/6 小鼠，每两天一次，持续两周。结果：FCCP 与 cisplatin 联合通过 OMA1 诱导线粒体和 ER 应激来抑制肿瘤生长。[4] |
| Cell experiments | Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times. (Only for Reference) |
| Synonyms | Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, Trifluoromethoxy carbonylcyanide phenylhydrazone |
| molecular weight | 254.17 |
| Molecular formula | C10H5F3N4O |
| CAS | 370-86-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 55 mg/mL (216.39 mM) |
| References | 1. Brennan JP, et al. FCCP is cardioprotective at concentrations that cause mitochondrial oxidation without detectable depolarisation. Cardiovasc Res. 2006 Nov 1;72(2):322-30. 2. Muñoz F, et al. Carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) induces initiation factor 2 alpha phosphorylation and translation inhibition in PC12 cells. FEBS Lett. 2001 Mar 9;492(1-2):156-9. 3. Grasmick KA, et al. Uncoupling of the Electron Transport Chain Compromises Mitochondrial Oxidative Phosphorylation and Exacerbates Stroke Outcomes. J Neuroinfect Dis. 2018;9(4):283. 4. Cheng M, et al. The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer. Int J Mol Sci. 2022 Jan 25;23(3):1320. |
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