| Description | Endosidin-2 is an exocyst inhibitor, a cell-permeable benzylidene benzoylhydrazine, that binds to the exocyst component of the 70 kDa (EXO70) subunit of the exocyst complex (Kd = 253 μM, EXO70A1).Endosidin-2 disrupts protein translocation between the endosomes and plasma membrane, thereby facilitating translocation of proteins to the vesicle for degradation. It also inhibits recirculation of endocytosed transferrin to the plasma membrane in HeLa cells and targets multiple isoforms of mammalian EXO70, leading to dysregulation of exocytosis.Endosidin2 can be used as a tool molecule to study cytosolization. |
| In vitro | Endosidin2 (ES2) 被用于研究苔藓植物Physcomitrium patens的极化细胞生长，其中植物暴露在不断增加的ES2浓度下，IC50在8.8至12.3 µM之间。当浓度达到50 µM时，尖端生长的细胞在其尖端附近破裂，这表明ES2通过抑制新的细胞壁材料通过胞吐作用的沉积来发挥作用。[2] |
| Target activity | Polarized cells:8.8 -12.3 µM |
| Synonyms | ES2, Endosidin 2 |
| molecular weight | 414.17 |
| Molecular formula | C15H12FIN2O3 |
| CAS | 1839524-44-5 |
| Storage | |Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 90.0 mg/mL (217.3 mM), Sonication is recommended. DMF: 30 mg/mL Ethanol: 0.25 mg/mL |
| References | 1. Zhang C, et al. Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis. Proc Natl Acad Sci U S A. 2016;113(1):E41-E50. 2. Bormann E, et al. Inhibition of the Exocyst Complex with Endosidin 2 Reduces Polarized Growth in Physcomitrium patens. MicroPubl Biol. 2022;2022:10.17912/micropub.biology.000655. |