| Description | DORA-22 is a dual orexin receptor antagonist, improves mild stress-induced insomnia with minimal effect on memory |
| In vivo | DORA-22, a dual orexin receptor antagonist, improves mild stress-induced insomnia with minimal effect on memory.DORA-22 treatment improved the insomnia-related sleep disruption-wake was attenuated and NREM sleep was normalized.?REM sleep amounts were enhanced compared with vehicle treatment for one dose (30 mg/kg).?In the first hour of insomnia model exposure, DORA-22 promoted the number and average duration of NREM sleep spindles, which have been previously proposed to play a role in memory consolidation (all doses).?Water maze measures revealed probe trial performance improvement for select doses of DORA-22, including increased time spent in the platform quadrant (10 and 30 mg/kg) and time spent in platform location and number of platform crossings (10 mg/kg only).?In conclusion, DORA-22 treatment improved insomnia-related sleep disruption and memory consolidation deficits[1]. |
| Animal experiments | Animals were first trained to remember the location of a hidden platform (acquisition) in the Morris Water Maze and then administered DORA-22 (10, 30, or 100 mg/kg doses) or vehicle control.?Animals were then subjected to a rodent insomnia model involving two exposures to dirty cages over a 6-hr time period (at time points 0 and 3 hr), followed immediately by a probe trial in which memory of the water maze platform location was evaluated[1]. |
| molecular weight | 424.44 |
| Molecular formula | C23H22F2N4O2 |
| CAS | 1088991-95-0 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 10 mg/mL (23.56 mM) |
| References | 1. Gamble M C , Katsuki F , Mccoy J G , et al. The dual orexinergic receptor antagonist DORA-22 improves the sleep disruption and memory impairment produced by a rodent insomnia model[J]. Sleep, 2019. 2. Duncan M J , Farlow H , Tirumalaraju C , et al. Effects of the dual orexin receptor antagonist DORA-22 on sleep in 5XFAD mice[J]. Alzhmer's & Dementia: Translational Research & Clinical Interventions, 2019, 5:70-80. |