| Description | Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) is a fluorescent probe for measuring oxidative stress in cell-free systems and cellular models. |
| In vitro | Dihydrofluorescein diacetate能够检测线粒体中活性氧(ROS)的存在。该化合物的荧光清晰地勾勒出与TMRM染色的线粒体在所有细节上一致的纤维结构。它进入线粒体并与基质中释放的ROS发生反应[1]。Dihydrofluorescein diacetate可能是量化测量经纳米颗粒处理的细胞氧化潜力的有用方法[2]。在再氧化的内皮中，Dihydrofluorescein diacetate显示与线粒体一致的线性结构的荧光[3]。 |
| Synonyms | 二氢荧光素二乙酸酯, Diacetyldihydrofluorescein |
| molecular weight | 418.4 |
| Molecular formula | C24H18O7 |
| CAS | 35340-49-9 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 100 mg/mL (239.01 mM) |
| References | 1. Diaz G, et al. Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes. Histochem Cell Biol. 2003 Oct;120(4):319-25. 2. Aranda A, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63. 3. Hempel SL, et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med. 1999 Jul;27(1-2):146-59. |