| Description | Dihydroethidium (PD-MY 003) is a peroxide indicator with blue fluorescence in the cytoplasm (λex=518 nm, λem=616 nm), which disappears when oxidized and is cell permeable. |
| In vitro | 方法: Dihydroethidium 检测细胞过氧化物：1、用 DMSO 配制 5-10 mM 的 Dihydroethidium 染料储备液，避光，分装储存于 -20℃。2、使用时用生理缓冲液 (如 PBS、HBSS、HEPES) 稀释储备液，得到 5-20 µM Dihydroethidium 染料工作液。最佳工作浓度必须根据经验确定。3、将染料工作液按 1:1 的比例加入到细胞中 (100 µL 细胞悬液加入 100 µL 染料工作液)，在室温或 37℃ 下孵育 5-60 分钟。4、用 PBS 洗涤后，进行荧光显微镜或流式细胞仪检测。Dihydroethidium 本身为蓝色荧光，最大激发波长为 370 nm，最大发射波长为 420 nm，脱氢后与 RNA 或 DNA 结合产生红色荧光，最大激发波长为 300 nm，最大发射波长为 610 nm，实际观察时也可以使用 518 nm 作为激发波长。 |
| Synonyms | 二氢乙锭, PD-MY 003, Hydroethidine |
| molecular weight | 315.41 |
| Molecular formula | C21H21N3 |
| CAS | 104821-25-2 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 5 mg/mL (15.85 mM) |
| References | 1. Zielonka J, et al. J Biol Chem. 2012 Jan 27;287(5):2984-95. 2. Liu H W, Luo Y, Zhou Y F, et al. Probucol Prevents Diabetes-Induced Retinal Neuronal Degeneration through Upregulating Nrf2[J]. BioMed Research International. 2020, 2020. |
| Citations | 1. Li J, Gao Y, Cui L, et al. Combination of monoammonium glycyrrhizinate and cysteine hydrochloride protects mice against acetaminophen-induced liver injury via Keap1/Nrf2/ARE pathway. Journal of Pharmacy and Pharmacology. 2022 2. Liu H W, Luo Y, Zhou Y F, et al. Probucol Prevents Diabetes-Induced Retinal Neuronal Degeneration through Upregulating Nrf2. BioMed Research International. 2020 3. Liu T, Zhang T, Guo C, et al.Murine double minute 2-mediated estrogen receptor 1 degradation activates macrophage migration inhibitory factor to promote vascular smooth muscle cell dedifferentiation and oxidative stress during thoracic aortic aneurysm progression.Biochimica et Biophysica Acta (BBA)-Molecular Cell Research.2024, 1871(3): 119661. |