| Description | DIF-3 is a chemical compound that acts by activating GSK-3β to facilitate the degradation of cyclin D1 and c-Myc, resulting in reduced expression levels of these proteins. In addition, DIF-3 inhibits Wnt/β-catenin signaling pathway-related proteins in DLD-1 cells. This compound exerts a potent antiproliferative effect on the HeLa human cervical cancer cell line by inducing cyclin D1 degradation and inhibiting cyclin D1 mRNA expression [1]. |
| In vitro | 在本研究中，我们探究了六种DIF类似物（DIF-1，DIF-2（含有pentanone替代hexanone），DIF-3（DIF-1的去氯形式），2-MIDIF-1（DIF-1的2-甲氧基异构体），DMPH（DIF-3的去氯形式），以及THPH（DMPH的4-羟基替代物））对人类白血病K562细胞的DNA合成、细胞生长、红细胞分化以及胞质游离钙浓度（[Ca2+]i）的影响。研究发现DIF-3是其中最强的抗白血病剂，其对生长抑制、红细胞诱导和[Ca2+]i增加的有效性顺序分别是THPH。DIF-3通过激活GSK-3beta加速cyclin D1的蛋白质降解，这一机制参与了DIF-3诱导的哺乳动物细胞G(0)/G(1)阶段阻滞。 |
| molecular weight | 272.72 |
| Molecular formula | C13H17ClO4 |
| CAS | 113411-17-9 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 90.0 mg/mL (330.0 mM), Sonication is recommended. |
| References | 1. Kubohara Y. Effects of differentiation-inducing factors of Dictyostelium discoideum on human leukemia K562 cells: DIF-3 is the most potent anti-leukemic agent. Eur J Pharmacol. 1999;381(1):57-62. 2. Takahashi-Yanaga F, et al. Dictyostelium differentiation-inducing factor-3 activates glycogen synthase kinase-3beta and degrades cyclin D1 in mammalian cells. J Biol Chem. 2003;278(11):9663-9670. |
| Citations | 1. Zheng C, Wang Y, Bi B, et al.Gallic acid ameliorates endometrial hyperplasia through the inhibition of the PI3K/AKT pathway and the down-regulation of cyclin D1 expression.Journal of Pharmacological Sciences.2024 |