| Description | Cycloleucine is an antagonist of NMDA receptor associated glycine receptor with a Ki of 600 μM. Cycloleucine is also a competitive inhibitor of S-adenosyl-methionine mediated methylation with anxiolytic and cytostatic effects. |
| In vitro | Cycloleucine (10 µg/mL) 有效抑制人类KB细胞系和小鼠L1210s白血病细胞系的活性。Cycloleucine (4-40 mM; 3小时) 阻断了B77转化鸡胚成纤维细胞内病毒RNA的内部甲基化。Cycloleucine (40 mM; 24小时) 通过超过90%阻断B77 38S RNA亚单位中m6A和倒数第二个Gm的形成[2]。 |
| In vivo | Cycloleucine（0.5、1.0、2.0、4 µg/µL；脑室内）在大鼠中增加了在开放臂时间、进入开放臂的次数以及极端到达的频率[3]。在Semliki Forest 病毒A7(74)株感染和对照小鼠中，Cycloleucine减少了胸腺和脾脏的重量[4]。 |
| molecular weight | 129.16 |
| Molecular formula | C6H11NO2 |
| CAS | 52-52-8 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: 45 mg/mL (348.41 mM), Sonication is recommended. DMSO: < 1 mg/mL (insoluble or slightly soluble), Sonication is recommended. |
| References | 1. Caboche M, et, al. RNA methylation and control of eukaryotic RNA biosynthesis. Effects of cycloleucine, a specific inhibitor of methylation, on ribosomal RNA maturation. Eur J Biochem. 1977 Mar 15;74(1):19-29. 2. Dimock K, et, al. Cycloleucine blocks 5'-terminal and internal methylations of avian sarcoma virus genome RNA. Biochemistry. 1978 Aug 22;17(17):3627-32. 3. Gargiulo API, et, al. Effects of Cycloleucine in the Nucleus Accumbens Septi on the Elevated plus Maze Test in Rats. Neuropsychobiology. 2020;79(3):191-197. 4. S Amor, et al. The effect of cycloleucine on SFV A7(74) infection in mice. Br J Exp Pathol. 1987 Apr;68(2):225-35. |