| Description | CHAPS is a zwitterionic nondenaturing detergent for solubilizing membrane proteins. |
| In vitro | CHAPS洗涤剂对膜蛋白质是非变性的，能够溶解蛋白质、解聚蛋白-蛋白相互作用，并且电性中性。CHAPS在离子交换色谱和等电聚焦中也很有用，因为它是两性离子的，从pH 2到12之间不呈现净电荷。CHAPS的临界胶束浓度为6-10mM。 |
| molecular weight | 614.88 |
| Molecular formula | C32H58N2O7S |
| CAS | 75621-03-3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: 46 mg/mL (74.81 mM) |
| References | 1. Ofri D, Ritter AM, Liu YF et al. Characterization of solubilized opioid receptors: reconstitution and uncoupling of guanine nucleotide-sensitive agonist binding. J Neurochem. 1992 Feb;58(2):628-35. 2. Banerjee P, Joo JB, Buse JT, Dawson G. Differential solubilization of lipids along with membrane proteins by different classes of detergents. Chem Phys Lipids. 1995 Aug 1;77(1):65-78. 3. Pasquali C, Fialka I, Huber LA. Preparative two-dimensional gel electrophoresis of membrane proteins. Electrophoresis. 1997 Dec;18(14):2573-81. 4. Menshikova I, Menshikov E, Filenko N, Lyubchenko YL. Nucleosomes structure and dynamics: effect of CHAPS. Int J Biochem Mol Biol. 2011;2(2):129-137. 5. Krofli A, Sarac B, Beter-Roga M. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012 Jul 17;28( |