| Description | BIS-TRIS propane (BTP), a novel multidentate ligand of nickel- and cobalt-based spin clusters, is a metal chelator. BIS-TRIS propane is often used as a zwitterionic buffer for PCR and protein crystallization studies. |
| Synonyms | BTP |
| molecular weight | 282.33 |
| Molecular formula | C11H26N2O6 |
| CAS | 64431-96-5 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 10 mg/mL (35.42 mM), Sonication is recommended. H2O: 80 mg/mL (283.36 mM), Sonication is recommended. |
| References | 1. K A Eckert, et al. DNA polymerase fidelity and the polymerase chain reaction. PCR Methods Appl. 1991 Aug;1(1):17-24. 2. J R Wenner, et al. Buffer effects on EcoRV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage. Anal Biochem. |