| Description | Ardisiacrispin A (Deglucocyclamin) has cytotoxic activity toward HepG2 cancer cell with the GI(50) value of 1.56μM, it could inhibit the proliferation of Bel-7402 cells by inducing apoptosis and disassembling microtubule.Ardisiacrispin(A+B) exhibit prominent abilities to inhibit the proliferation of HL-60 cells by blocking the cell cycle at S phase and inducing apoptosis. |
| In vitro | Ardisiacrispin A+Ardisiacrispin B是由Ardisia crenata提取，并以固定比例(2:1)混合的Ardisiacrispin A和ardisiacrispin B。本研究旨在探究其对人类癌细胞的抗癌活性及作用机制。通过基于sulphorhodamine B的比色分析法，发现Ardisiacrispin A+Ardisiacrispin B对几种人类癌细胞系生长的(IC50)s范围为0.9-6.5 microg/ml，其中Bel-7402细胞线最为敏感。此外，Ardisiacrispin A+Ardisiacrispin B能够在1-10 microg/ml的剂量下通过流式细胞仪诱导Bel-7402细胞剂量依赖性的凋亡，并通过高内容筛选分析导致线粒体膜去极化、膜通透性增强和核凝聚等变化。进一步研究发现，Ardisiacrispin A+Ardisiacrispin B可在5-20 microg/ml的浓度下破坏Bel-7402细胞中的微管结构，微管的荧光强度减弱。综上所述，Ardisiacrispin A+Ardisiacrispin B通过诱导凋亡和破坏微管结构抑制Bel-7402细胞的增殖。 |
| Synonyms | 百两金素, Saxifragifolin B, LTS-4, Deglucocyclamin |
| molecular weight | 1061.21 |
| Molecular formula | C52H84O22 |
| CAS | 23643-61-0 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 10.61 mg/mL (10 mM) |
| References | 1. Pro-apoptotic and microtubule-disassembly effects of ardisiacrispin (A+B), triterpenoid saponins from Ardisia crenata on human hepatoma Bel-7402 cells. J Asian Nat Prod Res. 2008 Jul-Aug;10(7-8):739-46. |