| Description | Amygdalin (Laetrile) has antifibrotic, antitumor, anti-inflammatory and analgesic effects, amygdalin joint HSYA could inhibit the degeneration of the endplate chondrocytes derived from intervertebral discs of rats induced by IL-1beta and better than the single use of Amygdalin or HSYA. Amygdalin induces apoptotic cell death in human DU145 and LNCaP prostate cancer cells by caspase-3 activation through down-regulation of Bcl-2 and up-regulation of Bax. |
| In vitro | Amygdalin具有抗肿瘤活性，已在其抗肿瘤机制方面取得一定进展[1]。特别是，Amygdalin下调属于细胞周期类别的基因：exonuclease 1、ATP-binding cassette，sub-family F，member 2、MRE11 meiotic recombination 11 homolog A、topoisomerase (DNA) I 以及 FK506 binding protein 12-rapamycin-associated protein 1。RT-PCR分析显示，这些基因的mRNA水平在SNU-C4人结肠癌细胞中经Amygdalin处理后也降低[2]。 |
| In vivo | Amygdalin在缓解炎症性疼痛方面有效，可作为具有抗伤害感受和抗炎活性的镇痛剂。肌肉注射Amygdalin显著降低了甲醛引发的持续性疼痛，在早期(甲醛注射后最初的10分钟)和晚期(甲醛初始注射后的10-30分钟)均有显著效果。Amygdalin以剂量依赖的方式减少了甲醛诱导的疼痛，在晚期剂量范围小于1 mg/kg时效果显著[3]。 |
| Cell experiments | Cell viability is determined by MTT assay. Cells are seeded in triplicate at a concentration of 1×105 cells/well on a 96-well plate. SNU-C4 cells are treated with amygdalin at concentrations of 0.25, 0.5, 2.5, and 5 mg/mL for 24 h. After MTT is added to each group, the cells are incubated for 4 h. Then, they are further incubated for 1 h, including the solution in which MTT is dissolved[2]. |
| Synonyms | 苦杏仁苷, Laetrile |
| molecular weight | 457.43 |
| Molecular formula | C20H27NO11 |
| CAS | 29883-15-6 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 60 mg/mL (131.17 mM) H2O: 45.74 mg/mL (100 mM), Sonication is recommended. Ethanol: < 1 mg/mL (insoluble or slightly soluble) |
| References | 1. Song Z, et al. Advanced research on anti-tumor effects of amygdalin. J Cancer Res Ther. 2014 Aug;10 Suppl 1:3-7. 2. Park HJ,et al. Amygdalin inhibits genes related to cell cycle in SNU-C4 human colon cancer cells. World J Gastroenterol. 2005 Sep 7;11(33):5156-61. 3. Hwang HJ,et al. Antinociceptive effect of amygdalin isolated from Prunus armeniaca on formalin-induced pain in rats. Biol Pharm Bull. 2008 Aug;31(8):1559-64. |