| Description | AkaLumine hydrochloride is a fluorescein analog with a Km value of 2.06 μM for recombinant fluorescent protein. |
| In vitro | 在LLC/luc和MDA-MB-231/Luc细胞中，经AkaLumine hydrochloride处理后，信号在较低浓度下(从2.5 μM开始)达到峰值。相反，由D-luciferin和CycLuc1产生的生物发光随着浓度的增加而持续上升，并且即便在最高浓度250 μM时，也没有达到明显的饱和点。[1] |
| In vivo | AkaLumine hydrochloride处理的小鼠，在静脉注射LLC/luc细胞后15分钟以5 mM的剂量，其在肺部检测散布癌细胞的敏感性相比实验对照组增加了3.3倍。相反地，以8小时的间隔，AkaLumine hydrochloride在肺转移信号上显示出相比实验对照组约四倍的增加。[1] |
| Target activity | Recombinant Fluc protein:2.06 μM |
| Synonyms | AkaLumine hydrochloride (1176235-08-7 free base), AkaLumine hydrochloride |
| molecular weight | 338.85 |
| Molecular formula | C16H19ClN2O2S |
| CAS | 2558205-28-8 |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 45.0 mg/mL (132.8 mM), Sonication is recommended. H2O: 45.0 mg/mL (132.8 mM);, Sonication is recommended. |
| References | 1. Kuchimaru T, et al. A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging. Nat Commun. 2016 Jun 14;7:11856. 2. Ogawa H, et al. Absorption Spectra for Firefly Bioluminescence Substrate Analog: TokeOni in Various pH Solutions. Photochem Photobiol. 2021;97(5):1016-1022. 3. Zambito G, et al. Evaluating Brightness and Spectral Properties of Click Beetle and Firefly Luciferases Using Luciferin Analogues: Identification of Preferred Pairings of Luciferase and Substrate for In Vivo Bioluminescence Imaging. Mol Imaging Biol. 2020;22(6):1523-1531. 4. Hendrickx S, et al. Comparison of Bioluminescent Substrates in Natural Infection Models of Neglected Parasitic Diseases. Int J Mol Sci. 2022;23(24):16074. |