| Description | Acetylcholine iodide (Acetylcolina) is a neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system. |
| In vitro | Acetylcholine iodide(Ach)在剂量依赖性的方式下促进了SBC3细胞向纤维连接蛋白(Fn)的增殖、黏附和迁移[1]。ACh通过降低p38-MAPK磷酸化和增强calpastatin表达，缓解了TNF-α诱导的calpain激活。它通过激活毒蕈碱型ACh受体(MAChR)及抗氧化系统发挥抗凋亡作用。ACh提高了H9c2细胞的活性，并减少了TNF-α诱导的细胞凋亡[2]。 |
| In vivo | Acetylcholine 能够抑制促炎细胞因子的释放，并保护心肌细胞免受损伤[2]。 |
| Cell experiments | The SBC3 cells are seeded at 2000 cells/well in treated 96-well plates maintained with RPMI1640 medium containing 10% (v/v) FCS for 24 h. The medium is removed and replaced with 100 μl RPMI1640 containing 1% (v/v) ITS. The cells are incubated in serum free medium for 24 h to synchronize the cell cycle. Then another 100 μl serum-free medium containing the agonist/antagonist under investigation is added. The antagonist is added 30 min before Ach(Acetylcholine iodide). At this time, the cells in a duplicate plate are given 100 μl serum-free medium and a MTT assay is used to determine live cell numbers before treatment with the agonist/antagonist as measured by the absorbance (OD) at 550 nm/650 nm. After 24, 48 or 72 h culture, the remaining cells are assayed to yield post-agonist/antagonist cell numbers using the same method. All the experiments are performed in triplicate.(Only for Reference) |
| Synonyms | Acetylcolina |
| molecular weight | 273.11 |
| Molecular formula | C7H16NO2·I |
| CAS | 2260-50-6 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 50 mg/mL (183.08 mM), Sonication is recommended. H2O: 33 mg/mL (120.83 mM) |
| References | 1. Zhang S, et al. Anticancer Res. 2010, 30(1):97-106. 2. Zhao M, et al. Cell Physiol Biochem. 2015, 36(5):1877-89. |