| Description | 7beta-Hydroxycholesterol, a major cytotoxin in oxidized LDL, induces Ca(2+) oscillations, MAP kinase activation and apoptosis in human aortic smooth muscle cells. |
| In vitro | 7beta-OHC decreased clonogenic survival of NCI-H460 in a dose dependent pattern. 7beta-OHC induced apoptosis in NCI-H460, with the characteristic features like increase in sub-G(1) hypodiploid (apoptotic) cells, and apoptotic body formation, as evidenced by flow cytometry and fluorescence microscope, respectively. Apoptosis was also associated with loss of mitochondrial transmembrane potential, and the activation of caspases 9 and 3. 7beta-OHC resulted in generation of reactive oxygen species (ROS) during apoptosis[1] |
| molecular weight | 402.65 |
| Molecular formula | C27H46O2 |
| CAS | 566-27-8 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| References | 1. Cytotoxic effect of 7beta-hydroxycholesterol on human NCI-H460 lung cancer cells.Biol. Pharm. Bull., 2005, 28(8):1377-80. |