7-Amino-4-methylcoumarin-3-acetic acid Bioactivity,Chemical Properties,Storage & Solubility Information Pathways-PeptideDB

Description	7-Amino-4-methylcoumarin-3-acetic acid (7-Amino-4-methylcoumarin) is more suitable as a substrate for enteropeptidase than GD(4)K-NA.
In vitro	在本研究中，我们采用了GD(4)K修饰的7-Amino-4-methylcoumarin（GD(4)K-AMC）发射光谱的变化作为一种检测肠道蛋白酶活性的荧光法。动力学分析表明，对于7-Amino-4-methylcoumarin，肠道蛋白酶的K(M)为0.025 mM，k(cat)为65 sec(-1)；而对于GD(4)K-NA，其K(M)为0.5至0.6 mM，k(cat)为25 sec(-1)。7-Amino-4-methylcoumarin水解的最适pH值为pH 8.0。
Synonyms	7-氨基-4-甲基-3-香豆素醋酸, 7-Amino-4-methylcoumarin
molecular weight	233.22
Molecular formula	C12H11NO4
CAS	106562-32-7
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility	DMSO: 55 mg/mL (235.83 mM)
References	1. A fluorogenic method for measuring enteropeptidase activity: spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin.BMB Rep. 2011 Jul;44(7):458-61.

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