| Description | 6-CFDA N-succinimidyl ester (Carboxyfluorescein diacetate succinimidyl) is capable of spontaneously and irreversibly binding to free amines. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells. |
| Synonyms | Carboxyfluorescein diacetate succinimidyl |
| molecular weight | 557.46 |
| Molecular formula | C29H19NO11 |
| CAS | 150206-15-8 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 10 mg/mL (17.94 mM) |
| References | 1. Stich S,etal.Regenerative and immunogenic characteristics of cultured nucleus pulposus cells from human cervical intervertebral discs.PLoS One. 2015 May 19;10(5):e0126954. 2. Yang T,etal.A novel nonradioactive CFDA assay to monitor the cellular immune response in myeloid leukemia.Immunobiology. 2013 Apr;218(4):548-53. 3. Urbani S,etal.Use of CFDA-SE for evaluating the in vitro proliferation pattern of human mesenchymal stem cells.Cytotherapy. 2006;8(3):243-53. |