| Description | 4-Azido-L-phenylalanine (p-Azido-L-phenylalanine) is an amino acid derivative that acts as a photoaffinity marker and can be used to detect localized protein environments. |
| In vitro | 在无细胞蛋白质合成（CFPS）方法中，CFPS溶液中累积的修饰可溶性超级折叠绿色荧光蛋白（sfGFP）的浓度范围为0.9至1.7 mg/mL，包括4-Azido-L-phenylalanine或p-propargyloxy-l-phenylalanine（pPaF）在内。[1] |
| Synonyms | p-Azidophenylalanine, p-Azido-L-phenylalanine |
| molecular weight | 206.2 |
| Molecular formula | C9H10N4O2 |
| CAS | 33173-53-4 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: 22.5 mg/mL (109.1 mM), when pH is adjusted to 11 with NaOH. Sonication is recommended. DMSO: 4.5 mg/mL (21.8 mM), when pH is adjusted to 3 with HCl. Sonication and heating to 80℃ are recommended. |
| References | 1. Albayrak C, et al. Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation. Nucleic Acids Res. 2013 Jun;41(11):5949-63. 2. Zhang J, et al. Identifying and Modulating Accidental Fermi Resonance: 2D IR and DFT Study of 4-Azido-l-phenylalanine. J Phys Chem B. 2018;122(34):8122-813 |