| Description | 3-Amino-1,2,4-triazole (Trapidil impurity B) is an inhibitor of catalase, capable of inducing the compensatory mechanism for hydrogen peroxide detoxification. This mechanism involves increasing the activities of glutathione peroxidase and glutathione reductase. |
| In vitro | 3-Amino-1,2,4-triazole (10 mM; 24h) treatment decreased CAT activity and increased intracellular H2O2 levels significantly. Glutathione peroxidase (GPX) activity was also reduced, and the gene expression levels of the antioxidant enzymes GPX4 and peroxiredoxins were inhibited.[5] |
| Synonyms | Amitrole, 3-AT, 1H-1,2,4-Triazol-5-amine, Trapidil impurity B |
| molecular weight | 84.08 |
| Molecular formula | C2H4N4 |
| CAS | 61-82-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 90.0 mg/mL (1070.4 mM), Sonication is recommended. |
| References | 1. Pan H, et al. Mechanism of the effects of amitrole on the thyroglobulin in Fischer rat thyroid follicle-5 cell. Wei Sheng Yan Jiu. 2011;40(4):434-440. 2. Pan HM, et al. Effects of amitrole on thyroid hormone-associated gene transcription in FRTL-5 cells. Nan Fang Yi Ke Da Xue Xue Bao. 2008;28(1):12-15. 3. Furukawa A, et al. Oxidatively generated DNA damage induced by 3-amino-5-mercapto-1,2,4-triazole, a metabolite of carcinogenic amitrole. Mutat Res. 2010;694(1-2):7-12. 4. La Rocca N, et al. Amitrole treatment of etiolated barley seedlings leads to deregulation of tetrapyrrole synthesis and to reduced expression of Lhc and RbcS genes. Planta. 2001;213(1):101-108. 5. Ruiz-Ojeda F J, et al. Impact of 3-amino-1, 2, 4-triazole (3-AT)-derived increase in hydrogen peroxide levels on inflammation and metabolism in human differentiated adipocytes. PLoS One, 2016, 11(3): e0152550. |