| Description | 2-NBDG is a fluorescent indicator (λex=467 nm, λem=542 nm) and a fluorescent D-glucose analog. 2-NBDG can be used to monitor glucose uptake in living cells and tissues by real-time confocal, high-resolution, or wide-field fluorescence microscopy and flow cytometry. |
| In vitro | 方法：Flow cytometry 检测葡萄糖摄取：1、细胞按 1*104/孔种于96孔板，24-48 h 内进行实验。2、去除细胞培养基，加入含 2-NBDG (5-40 µM) 的新鲜培养基，在37 ℃ 孵育 10-180 min。3、去除培养基，用预冷的 PBS 洗两次，2-NBDG 摄取反应停止。重悬于新鲜培养基，在30 min 内进行流式细胞术检测。[1] |
| In vivo | 方法：fluorescent microscope 检测循环乳腺癌细胞的葡萄糖摄取：1、通过穿刺小鼠隐静脉收集小鼠血液样本 (100 µL/mouse)。2、将含有循环乳腺癌细胞的血液样品与 2-NBDG (5 µg/100 µL 血液) 在 37℃ 的黑暗培养箱中孵育 30 min。3、将磁珠悬浮液 (1µL 1% )添加到 100 µL 的血液样品中，然后在 4℃ 下孵育 30 min，轻轻摇晃，以促进磁珠与循循环乳腺癌细胞结合。4、用磁性分离架分离从血液中收集循环的乳腺癌症细胞，用 PBS 洗涤 3 次，再次悬浮在 100 µL PBS 中后转移到 96 孔细胞板中。5、在配备 488 nm 滤光片的荧光显微镜下检查循环乳腺癌细胞对 2-NBDG 的摄取。具有来源于荧光 2-NBDG 的细胞摄取的荧光信号的大细胞被计数为高代谢循环乳腺癌症细胞，小尺寸的正常小鼠血细胞 (淋巴细胞和 RBC) 没有或很少显示 2-NBDG 荧光信号。[2] |
| Synonyms | 2-(N-7-硝基-2,1,3-苯并恶二唑-4-氨基)-2-脱氧-D-葡萄糖 |
| molecular weight | 342.26 |
| Molecular formula | C12H14N4O8 |
| CAS | 186689-07-6 |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: 5 mg/mL (14.61 mM), Sonication and heating to 60℃ are recommended. |
| References | 1. Zou C, et al. 2-NBDG as a fluorescent indicator for direct glucose uptake measurement. J Biochem Biophys Methods. 2005 Sep 30;64(3):207-15. 2. Cai H, et al. 2-NBDG fluorescence imaging of hypermetabolic circulating tumor cells in mouse xenograft model of breast cancer. J Fluoresc. 2013 Jan;23(1):213-20. |
| Citations | 1. Zhang Y, Cai Y, Wang T, et al.A common tolerance mechanism of bacterial biofilms to antibiotics.bioRxiv.2023: 2023.01. 30.526163. 2. Ma Y, Pang Y, Cao R, et al.Targeting Parkin-regulated metabolomic change in cartilage in the treatment of osteoarthritis.iScience.2024 |