| Description | 2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein), a cell-permeable fluorogenic probe, is useful for the detection of nitric oxide (NO) and reactive oxygen species (ROS) and for the determination of the degree of overall oxidative stress. |
| Synonyms | Diacetyldichlorofluorescein, Leuco-dcf diacetate, Dcfh-DA, 双氯荧光黄乙酸乙酯, DCFDA |
| molecular weight | 485.27 |
| Molecular formula | C24H14Cl2O7 |
| CAS | 2044-85-1 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 5.79 mg/ml (11.93 mM), Sonication is recommended. |
| References | 1. Kaur M, Esau L. Two-step protocol for preparing adherent cells for high-throughput flow cytometry. Biotechniques. 2015 Sep 1;59(3):119-26. doi: 10.2144/000114325. eCollection 2015 Sep. PubMed PMID: 26345504. 2. Aranda A, Sequedo L, Tolosa L, Quintas G, Burello E, Castell JV, Gombau L. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63. doi: 10.1016/j.tiv.2013.01.016. Epub 2013 Jan 26. PubMed PMID: 23357416. 3. Mohan S, Thiagarajan K, Chandrasekaran R. In vitro evaluation of antiproliferative effect of ethyl gallate against human oral squamous carcinoma cell line KB. Nat Prod Res. 2015;29(4):366-9. doi: 10.1080/14786419.2014.942303. Epub 2014 Aug 7. PubMed PMID: 25104086. 4. Nanda SS, An SS, Yi DK. Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif. Int J Nanomedicine. 2015 Aug 25;10 Spec Iss:93-9. doi: 10.2147/IJN.S88378. eCollection 2015. PubMed PMID: 26347475; PubMed Central PMCID: PMC4554416. |
2',7'-Dichlorofluorescein diacetate
CAS No.: 2044-85-1
2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein), a cell-permeable fluorogenic probe, is useful for the
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