| Description | Tetrahydrofolic acid (5,6,7,8-tetrahydrofolic acid) is a vitamin B9 derivative that is a common one-carbon donor in organisms and is involved in growth and metabolism. |
| In vitro | 在 Adh5-/- DT40 细胞中，Tetrahydrofolic acid（0-200 µM；3 天）导致细胞存活率迅速下降[1]。 |
| In vivo | 在 Adh5-/- 小鼠中，Tetrahydrofolic acid （62.5 mg/kg；腹腔注射；每日一次）造成了造血功能紊乱，增加了 Ser139-H2AX 的磷酸化，降低了祖细胞（HSPCs）的存活率[1]。 |
| Synonyms | 5,6,7,8-tetrahydrofolic acid, folate-H4, Tetrahydropteroylglutamic acid, th-folate |
| molecular weight | 445.43 |
| Molecular formula | C19H23N7O6 |
| CAS | 135-16-0 |
| Storage | store under nitrogen,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 1 mg/mL (2.25 mM), Sonication is recommended. H2O: 1 mg/mL (2.25 mM), Sonication is recommended. |
| References | 1. Clara B García-Calderón, et al. Genotoxicity of Tetrahydrofolic Acid to Hematopoietic Stem and Progenitor Cells. Cell Death Differ. 2018 Nov;25(11):1967-1979. 2. Lin RL,et al. Tetrahydrofolic acid: an inhibitor of the methyltetrahydrofolic acid-mediated methylation of indolethylamines. Biochim Biophys Acta. 1975 Apr 7;385(2):268-74. 3. Zhang M,et al. The Second Class of Tetrahydrofolate (THF-II) Riboswitches Recognizes the Tetrahydrofolic Acid Ligand via Local Conformation Changes. Int J Mol Sci. 2022 May 25;23(11):5903. 4. Natsuhori M,et al. Tetrahydrofolic acid as the principal congener of plasma folates in pigs. Am J Physiol. 1991 Jul;261(1 Pt 2):R82-6. |