| Description | Sedanolide is a natural phthalide first isolated from seed oil of the Umbelliferae family, including celery. |
| In vitro | Sedanolide suppressed J5 cell viability by inducing autophagy. Phosphoinositide 3-kinase (PI3K)-I, mammalian target of rapamycin (mTOR) and Akt protein levels decreased, whereas PI3K-III, LC3-II and Beclin-1 protein levels increased following Sedanolide treatment in J5 cells. In addition, Sedanolide treatment upregulated nuclear p53 and damage-regulated autophagy modulator (DRAM) and downregulated cytosolic p53 and Tp53-induced glycolysis and apoptosis regulator (TIGAR) expression in J5 cells. Furthermore, the cytosolic phosphorylation of inhibitor of kappa B (IκB) and nuclear p65 and the DNA-binding activity of NF-κB increased after Sedanolide treatment. These results suggest that Sedanolide induces J5 cell autophagy by regulating PI3K, p53 and NF-κB autophagy-associated signaling pathways in J5 cells. |
| molecular weight | 194.27 |
| Molecular formula | C12H18O2 |
| CAS | 6415-59-4 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 9 mg/mL (46.33 mM) |
| References | 1. Hsieh S L , Chen C T , Wang J J , et al. Sedanolide induces autophagy through the PI3K, p53 and NF-κB signaling pathways in human liver cancer cells[J]. International Journal of Oncology, 2015, 47(6). |