| Description | Rubrofusarin gentiobioside (Rubrofusarin-6-O-beta-D-gentiobioside) can significantly decrease the expression of TGF-beta1 and fibronectin and NF-kappaB DNA binding activity, suggests that it has potential as a preventive agent for advanced glycation end products-related diabetic complications. |
| In vitro | 为了研究CS的丁醇可溶提取物在糖尿病肾病条件下的药理效应，我们评估了在S100b（高级糖化终产品受体的特异性配体）存在下培养的小鼠肾小球系膜细胞中转化生长因子-β1（TGF-β1）和纤维连接蛋白表达情况。这些是糖尿病肾病的关键介质。CS抑制了S100b诱导的小鼠系膜细胞中TGF-β1和纤维连接蛋白的表达，通过抑制Smad2/3、细胞外信号调节激酶（ERK）/丝裂原激活蛋白激酶（MAPK）及氧化应激的活化实现。此外，CS还抑制了在S100b刺激下小鼠系膜细胞中核因子-κB (NF-κB)的激活。为鉴定CS中的活性化合物，对三种主要化合物，Rubrofusarin-6-O-β-D-gentiobioside (CS-A)、toralactone-9-O-β-D-gentiobioside (CS-B)和cassiaside (CS-C)在细胞中进行了测试。结果表明，CS-A显著降低了TGF-β1和纤维连接蛋白的表达及NF-κB DNA结合活性。 |
| Synonyms | Rubrofusarin-6-O-beta-D-gentiobioside, 红链霉素-龙胆二糖苷, 芸苔红素龙胆苷 |
| molecular weight | 596.53 |
| Molecular formula | C27H32O15 |
| CAS | 24577-90-0 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 22.5 mg/mL (37.72 mM) |
| References | 1. Extract of Cassiae Semen and its major compound inhibit S100b-induced TGF-beta1 and fibronectin expression in mouse glomerular mesangial cells.Eur J Pharmacol. 2010 Sep 1;641(1):7-14. |