| Description | Ecdysone is a major steroid hormone in insects and herbs. Ecdysone triggers mineralocorticoid receptor activation and induces cellular apoptosis. Ecdysone signaling through Ecdysone receptor isoform B1 is required cell autonomously for the muscle death. |
| In vitro | Ecdysone（10, 100 nM）诱导α-平滑肌肌动蛋白（SMA）的表达。以剂量依赖的方式，Ecdysone提升了裂解caspase 3的表达水平。Ecdysone（10, 100 nM）显著抑制了细胞移动性和划痕愈合能力[4]。Ecdysone（100 nM）诱导出树突状纺锤体的间充质样细胞形态。Ecdysone（100 nM）导致肾小管内髓集合管细胞发生凋亡[4]。 |
| In vivo | 在雄性C57BL/6小鼠中，Ecdysone（6 μg/g/天；皮下注射）显著损害了肾功能，并且BUN水平有统计学上的显著增加，放大了肾脏中α-SMA的表达。Ecdysone还展示了MR依赖的肾病效应[4]。 |
| molecular weight | 464.63 |
| Molecular formula | C27H44O6 |
| CAS | 3604-87-3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 60 mg/ml (129.14 mM) |
| References | 1. Gautam NK, Verma P, Tapadia MG. Ecdysone regulates morphogenesis and function of Malpighian tubules in Drosophila melanogaster through EcR-B2 isoform. Dev Biol. 2015 Feb 15;398(2):163-76. 2. Nishita Y. Ecdysone response elements in the distal promoter of the Bombyx Broad-Complex gene, BmBR-C. Insect Mol Biol. 2014 Jun;23(3):341-56. 3. Minglei Lu, et al. Activation of Mineralocorticoid Receptor by Ecdysone, an Adaptogenic and Anabolic Ecdysteroid, Promotes Glomerular Injury and Proteinuria Involving Overactive GSK3β Pathway Signaling. Sci Rep. 2018 Aug 15;8(1):12225. 4. Minglei Lu, et al. Ecdysone Elicits Chronic Renal Impairment via Mineralocorticoid-Like Pathogenic Activities. Cell Physiol Biochem. 2018;49(4):1633-1645. |