| Description | Bryonolic acid (20-epi-Bryonolic acid), a triterpenoid extracted from Sandoricum indicum, possesses immunomodulatory, anti-inflammatory, antioxidant and anticancer activities and protects PC12 cells from NMDA-induced apoptosis by inhibiting Ca2+ in-flux and modulating gene expression in the Ca2+-CaMKII-CREB signalling pathway. |
| In vitro | 在存在三种诱导剂(甲基茉莉酸酯、酵母提取物和壳聚糖)的情况下，经过8天处理1 mg/mL壳聚糖的细胞悬浮液导致更高的 Bryonolic acid 含量(23.56 ± 1.68 mg/g干重),具有高 Bryonolic acid 含量的细胞培养液和根提取物导致 Saos-2 和 MCF-7 细胞的细胞存活率明显高于观察到的对照组(1% v/v DMSO)处理[1]。Bryonolic acid 在完整的癌细胞中抑制ACAT，IC50为12.6 ± 2.4 µM。Bryonolic acid 抑制了几种癌细胞系的克隆形成能力和侵袭能力[2]。 |
| In vivo | 用500 mg/kg Bryonolic acid 或载体腹腔注射处理Nrf2野生型或Nrf2敲除小鼠，结果表明 Bryonolic acid 依赖于Nrf2-Keap1通路的方式有效诱导 HO-1[3]。 |
| Target activity | M1 mAChR:7.08 (pKi), M3 mAChR:6.70 (pKi), mAChR4:7.00 (pKi), H1 Receptor:7.48 (pA2), M2 mAChR:7.78 (pKi) |
| Synonyms | UNII-J7YR6A878I, 20-epi-Bryonolic acid |
| molecular weight | 456.7 |
| Molecular formula | C30H48O3 |
| CAS | 24480-45-3 |
| Storage | keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 1 mg/mL (2.19 mM), Sonication is recommended. |
| References | 1. Pornpatsorn Lertphadungkit, et al. Enhanced Production of Bryonolic Acid in Trichosanthes cucumerina L. (Thai Cultivar) Cell Cultures by Elicitors and Their Biological Activities. Plants (Basel). 2020 Jun 2;9(6):709. 2. Farid Khallouki, et al. Bryonolic Acid Blocks Cancer Cell Clonogenicity and Invasiveness through the Inhibition of Fatty Acid: Cholesteryl Ester Formation. Biomedicines. 2018 Feb 12;6(1):21. 3. Tonibelle N Gatbonton-Schwager , et al. Bryonolic acid transcriptional control of anti-inflammatory and antioxidant genes in macrophages in vitro and in vivo. J Nat Prod. 2012 Apr 27;75(4):591-8. 4. Barker EC, Gatbonton-Schwager TN, Han Y, Clay JE, Letterio JJ, Tochtrop GP. Bryonolic acid: a large-scale isolation and evaluation of heme oxygenase 1 expression in activated macrophages. J Nat Prod. 2010 Jun 25;73(6):1064-8. doi: 10.1021/np1000076. PubMed PMID: 20481554; PubMed Central PMCID: PMC2905313. |