| Description | 9-Methoxycamptothecin (MCPT) has antitumour activities through topoisomerase inhibition. |
| In vitro | 9-Methoxycamptothecin(MCPT) induced cytotoxicity in seven human cancer cell lines in a dose dependent manner after 72h, with A2780 and Hela cell lines more sensitive, so the two cell lines were chosen to do further studies. MCPT induced strong G2/M arrest in both A2780 cells and Hela cells after 24h, following by substantial sub-G1 arrest (indicating apoptosis). The apoptosis was verified by staining with Annexin V-FITC and propidium iodide. ROS generation increased significantly in MCPT-induced apoptosis. Meanwhile, the apoptosis appeared to be dependent on caspase-3, -8 and -9 in A2780 cells, and caspase-3 in Hela cells. In addition, MCPT induced up-regulation expression of most of seventeen genes in both cell lines. Western blot verified that changes of TNFα, Fas, P53 and P27 protein level were consistent with their gene expression changes[1]. |
| molecular weight | 378.38 |
| Molecular formula | C21H18N2O5 |
| CAS | 39026-92-1 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 50 mg/mL (132.14 mM) |
| References | 1. Wang H , Ao M , Wu J , et al. TNFα and Fas/FasL pathways are involved in 9-Methoxycamptothecin-induced apoptosis in cancer cells with oxidative stress and G2/M cell cycle arrest[J]. Food and Chemical Toxicology, 2013, 55(Complete):396-410. 2. Liao N , Zhang P , Ao M , et al. 9-Methoxycamptothecin from Nothapodytes foetida Induces Apoptosis in Murine Sarcoma S180 Cells[J]. Zeitschrift für Naturforschung C, 2011, 66(9-10):471---476. |