3-(3-Hydroxyphenyl)propionic Acid-product

Description	3-(3-Hydroxyphenyl)propionic Acid (m-Hydroxyphenylpropionic acid) is one of the major metabolites of ingested caffeic acid.it inhibited osteoclastogenesis and bone osteoclastic resorptive activity.
In vitro	3-(3-hydroxyphenyl) propionic acid (3-3-PPA) and hippuric acid (HA), had dose-dependent suppressive effects on osteoclastogenesis and osteoclast resorptive activity in Corning osteo-assay plates.?Signaling pathway analysis showed that after pretreatment with HA or 3-3-PPA, RANKL-stimulated increase of osteoclastogenic markers, such as nuclear factor of activated T-cells, cytoplasmic 1 and matrix metallopeptidase 9 gene/protein expression were blunted.?Inhibitory effects of HA and 3-3-PPA on osteoclastogenesis utilized RANKL/RANK independent mediators[1].
Cell experiments	RAW264.7 cells or nonadherent bone marrow cells were cultured in 96‐well plates (2?×?10^4cells/well) in the presence or absence of 50?ng/ml of RANKL.?Cells were treated with HA at four different concentrations, 0.01×, 0.1×, 1×, and 10×, with 1× equivalent to concentration of 60?μg/dl of HA free form appeared in blood after 10% BB supplemental diet in rats.?Four different concentrations of 3‐3‐PPA or 3‐(4‐hydroxyphenyl) propionic acid (3–4‐PPA), 0.1×, 1×, 10×, and 100×, with 1× equivalent to concentration of 10?μg/dl of 3‐3‐PPA or 3–4‐PPA free form appeared in blood after 10% BB supplemental diet in rats.?After 4 days for RAW264.7 cell cultures and 5 days for bone marrow cell cultures, the cells were ?xed with 4% paraformaldehyde and stained for tartrate‐resistant acid phosphatase (TRAPase) activity using a TRAPase Staining Kit .?TRAP‐positive cells containing >3 nuclei in each well were counted as osteoclasts under an epifluorescent microscope[1]
Synonyms	m-Hydroxyphenylpropionic acid, 3-Hydroxyhydrocinnamic acid, 3-(3-羟基苯基)丙酸
molecular weight	166.17
Molecular formula	C9H10O3
CAS	621-54-5
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year
Solubility	DMSO: 33mg/mL(198.59mM)
References	1. Zhao H , Lazarenko O P , Jin‐Ran Chen. Hippuric acid and 3‐(3‐hydroxyphenyl) propionic acid inhibit murine osteoclastogenesis through RANKL‐RANK independent pathway[J]. Journal of Cellular Physiology, 2020, 235(1). 2. Najmanová, Iveta, Pourová, Jana, Vopr?alová, Marie, et al. Flavonoid metabolite 3-(3-hydroxyphenyl)propionic acid formed by human microflora decreases arterial blood pressure in rats[J]. Molecular Nutrition & Food Research, 2016, 60(5):981-991.

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3-(3-Hydroxyphenyl)propionic Acid

CAS No.: 621-54-5